Research on the fermentation of carica papaye vinegar by shake - flask culture 釀制木瓜果醋的研究
The vhb expression in the strain hv was investigated by co - binding assay . in flask culture , the strain hv had almost cell densities compared with e . colihms174 in the early phase of the growth 在搖瓶中培養(yǎng),發(fā)現(xiàn)在培養(yǎng)前期, hv的生長與無vhb基因的對照菌e . colihms174相差不多,到培養(yǎng)后期時, vhb基因整合菌hv才略有生長優(yōu)勢。
It is the foundation of therapy of hepatitis b . the gene of scfv was cloned into the expression vector pet32a ( + ) and subsequently expressed in escherichia coll . the expressed scfv fused with the 109 aa trx - tag thioredoxin protein was above 30 % total cell protein in the shaking flask cultures 融合硫氧還蛋白的單鏈抗體在大腸桿菌bl21 ( de3 )中以包涵體的形式得到高效表達,單鏈抗體表達量達占30的總蛋白以上。
Shake - flask culture condition of lysine fermentation were studied with czl . appropriate feeding of organic niteogen sources was favorable for fermentation jn our experiment , the concentations of the yeast extract and beef extract were 15g / l and 25g / l . the initial ( nh02so4 and glucose concentrations suitable for fermentor production ranged form 55g / l and 150 - 180g / l respectively 通過搖瓶發(fā)酵實驗初步確定czl的發(fā)酵條件為:初糖: 150 - 180g / l ,采用無機氮源和有機氮源配和使用,最佳組合是:硫酸銨質量濃度為55g / l ;酵母膏質量濃度為15g / l ,牛肉膏質量濃度為25g / l為最佳。